Monday, June 03, 2013

RNA sample quality assessment

RIN: http://www.biomedcentral.com/1471-2199/7/3

The RQN is equivalent to the broadly accepted RIN and is based on a proprietary algorithm that uses three areas of the electropherogram: (A) the area before the 18S peak, (B) the total area of the 18S and 28S peaks, and (C) the ratio of the 28S and 18S peaks. (Reference: http://www.genengnews.com/gen-articles/simplifying-ht-rna-quality-quantity-analysis/4688/)

RIN is highly correlated with RQN:

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